Breakpoint regions of ETO gene involved in (8; 21) leukemic translocations are enriched in acetylated histone H3. Issue 11 (13th September 2013)

• Record Type: Journal Article
• Authors: Stuardo, Marcela; Nicovani, Sandra; Javed, Amjad; Gutierrez, Soraya
• Is Part Of: Journal of cellular biochemistry. Volume 114:Issue 11(2013:Nov.)
• Page Start: 2569
• Page End: 2576
• Publication Date: 2013-09-13
• Subjects: Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.24605
• Languages: English
• ISSNs: 0730-2312

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jcb24605-sec-0001" sec-type="section"> <p>One of the most frequent chromosomal translocation found in patients with acute myeloid leukemia (AML) is the t(8;21). This translocation involves the <italic>RUNX1</italic> and <italic>ETO</italic> genes. The breakpoints regions for t(8;21) are located at intron 5 and intron 1 of the <italic>RUNX1</italic> and <italic>ETO</italic> gene respectively. To date, no homologous sequences have been found in these regions to explain their recombination. The breakpoint regions of <italic>RUNX1</italic> gene are characterized by the presence of DNasaI hypersensitive sites and topoisomerase II cleavage sites, but no information exists about complementary regions of <italic>ETO</italic> gene. Here, we report analysis of chromatin structure of <italic>ETO</italic> breakpoint regions. Chromatin immunoprecipitation (ChIP) were performed with antibodies specific to acetylated histone H3, H4, and total histone H1. Nucleosomal distribution at the <italic>ETO</italic> locus was evaluated by determining total levels of histone H3. Our data show that in myeloid cells, the breakpoint regions at the <italic>ETO</italic> gene are enriched in hyperacetylated histone H3 compared to a control region of similar size where no translocations have been described. Moreover, acetylated H4 associates with both the whole <italic>ETO</italic> breakpoint regions as well as the control intron. Interestingly, we observed no H1 association either at the breakpoint regions or the control region of the <italic>ETO</italic> gene. Our data indicate that a common chromatin structure enriched in acetylated histones is present in breakpoint regions involved in formation of (8;21) leukemic translocation. J. Cell. Biochem. 114: 2569–2576, 2013. © 2013 Wiley Periodicals, Inc.</p> </sec> </abstract>