Vitamin D Treatment Modulates Organic Dust–Induced Cellular and Airway Inflammatory Consequences. Issue 1 (20th December 2012)

• Record Type: Journal Article
• Authors: Golden, Gregory A.; Wyatt, Todd A.; Romberger, Debra J.; Reiff, Daniel; McCaskill, Michael; Bauer, Christopher; Gleason, Angela M.; Poole, Jill A.
• Is Part Of: Journal of biochemical and molecular toxicology. Volume 27:Issue 1(2013:Jan.)
• Page Start: 77
• Page End: 86
• Publication Date: 2012-12-20
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Toxicology -- Periodicals; 574
• DOI: 10.1002/jbt.21467
• Languages: English
• ISSNs: 1095-6670

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>Exposure to organic dusts elicits airway inflammatory diseases. Vitamin D recently has been associated with various airway inflammatory diseases, but its role in agricultural organic dust exposures is unknown. This study investigated whether vitamin D reduces organic dust–induced inflammatory outcomes in cell culture and animal models. Organic dust extracts obtained from swine confinement facilities induced neutrophil chemokine production (human IL‐8, murine CXCL1/CXCL2). Neutrophil chemokine induction was reduced in human blood monocytes, human bronchial epithelial cells, and murine lung slices pretreated with 1, 25‐(OH)₂D₃. Intranasal inhalation of organic dust extract induced neutrophil influx, and CXCL1/CXCL2 release was also decreased in mice fed a relatively high vitamin D diet as compared to mice fed a low vitamin D diet. These findings were associated with reduced tracheal epithelial cell PKCα and PKCε activity and whole lung TLR2 and TLR4 gene expression. Collectively, vitamin D plays a role in modulating organic dust–induced airway inflammatory outcomes. © 2012 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:77‐86, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21467</p> </abstract>