Whispering gallery mode aptasensors for detection of blood proteins. Issue 2 (28th March 2012)

• Record Type: Journal Article
• Authors: Pasquardini, Laura; Berneschi, Simone; Barucci, Andrea; Cosi, Franco; Dallapiccola, Ramona; Insinna, Massimiliano; Lunelli, Lorenzo; Conti, Gualtiero Nunzi; Pederzolli, Cecilia; Salvadori, Simone; Soria, Silvia
• Is Part Of: Journal of biophotonics. Volume 6:Issue 2(2013:Feb.)
• Page Start: 178
• Page End: 187
• Publication Date: 2012-03-28
• Subjects: Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201200013
• Languages: English
• ISSNs: 1864-063X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Whispering gallery mode resonators (WGMR), as silica microspheres, have been recently proposed as an efficient tool for the realisation of optical biosensors. In this work we present a functionalization procedure based on the DNA‐aptamer sequence immobilization on WGMR, able to recognize specifically thrombin or VEGF protein, preserving a high <italic>Q</italic> factor. The protein binding was optically characterized in terms of specificity in buffer solution or in 10% diluted human serum. Simulation of the protein flow was found in good agreement with experimental data. The aptasensor was also chemically regenerated and tested again, demonstrating the reusability of our system. (© 2013 WILEY‐VCH Verlag GmbH &amp; Co. KGaA, Weinheim)</p> </abstract>