Clerodendrumic Acid, a New Triterpenoid from Clerodendrum glabrum (Verbenaceae), and Antimicrobial Activities of Fractions and Constituents. Issue 9 (18th September 2013)

• Record Type: Journal Article
• Authors: Masevhe, Ndivhaleni A.; Awouafack, Maurice D.; Ahmed, Aroke S.; McGaw, Lyndy J.; Eloff, Jacobus N.
• Is Part Of: Helvetica chimica acta. Volume 96:Issue 9(2013:Sep.)
• Page Start: 1693
• Page End: 1703
• Publication Date: 2013-09-18
• Subjects: Chemistry -- Periodicals; Chemistry, Analytical -- periodicals; Chimie -- Périodiques; 540
• DOI: 10.1002/hlca.201200552
• Languages: English
• ISSNs: 0018-019X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>One new triterpenoid, (3<italic>β</italic>, 11<italic>α</italic>, 19<italic>β</italic>)‐3‐(butanoyloxy)‐11‐hydroxytaraxast‐20(30)‐ene‐23, 28‐dioic acid (clerodendrumic acid; <bold>1</bold>) was isolated from the hexane extract of the leaves of <italic>Clerodendrum glabrum</italic> var. <italic>glabrum</italic> along with heptadecanoic acid (<bold>2</bold>). The structure of the new compound was elucidated by interpretation of its NMR (1D and 2D), MS, and IR data. Combined fractions <italic>C</italic> and <italic>D</italic> from the column chromatography of the hexane extract exhibited significant antifungal activities (average <italic>MIC</italic> of 0.10 mg/ml) against <italic>Candida albicans</italic> and <italic>Cryptococcus neoformans. C. albicans</italic> was relatively resistant to clerodendrumic acid (<bold>1</bold>; <italic>MIC</italic> 125 μg/ml) and was resistant to heptadecanoic acid (<bold>2</bold>; <italic>MIC</italic> 188 μg/ml). Both compounds had low antibacterial activities against two <italic>Gram</italic>‐positive and two <italic>Gram</italic>‐negative bacteria with average <italic>MIC</italic> values of 157 and 172 μg/ml, respectively. Compounds <bold>1</bold> and <bold>2</bold> were relatively nontoxic against monkey kidney <italic>Vero</italic> cells <italic>in vitro</italic> with <italic>IC</italic>₅₀ values of 202.6 and 108.4 μg/ml, respectively.</p> </abstract>