Comparative functional cell biological analysis of mesenchymal stem cells of the head and neck region: Potential impact on wound healing, trauma, and infection. Issue 11 (14th November 2012)

• Record Type: Journal Article
• Authors: Jakob, Mark; Hemeda, Hatim; Bruderek, Kirsten; H. Gerstner, Andreas O.; Bootz, Friedrich; Lang, Stephan; Brandau, Sven
• Is Part Of: Head & neck. Volume 35:Issue 11(2013:Nov.)
• Page Start: 1621
• Page End: 1629
• Publication Date: 2012-11-14
• Subjects: Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.23196
• Languages: English
• ISSNs: 1043-3074

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="hed23196-sec-0001" sec-type="section"> <title>Background</title> <p>Mesenchymal stem cells (MSCs) are multipotent mesenchymal progenitor cells, originally identified in bone‐marrow. Little is known about MSCs of the head and neck region. We investigated cell biological properties with a potential impact on wound healing of 2 different tissue‐resident MSC populations.</p> </sec> <sec id="hed23196-sec-0002" sec-type="section"> <title>Methods</title> <p>MSCs were isolated from human nasal mucosa (nmMSCs) and parotid gland (pgMSCs). Clonogenic potential, cell surface markers, cytokine secretion, chemokine receptor expression, mobility, and adhesion to extracellular matrix were examined in unstimulated and stimulated MSCs.</p> </sec> <sec id="hed23196-sec-0003" sec-type="section"> <title>Results</title> <p>NmMSCs had the higher clonogenic potential. PgMSCs showed a broader panel of chemokine receptor expression and displayed higher mobility, especially after challenge with bacterial lipopolysaccharide (LPS). NmMSCs were less mobile and showed increased LPS‐induced secretion of the inflammatory cytokine interleukin‐8 (IL‐8) compared with pgMSCs.</p> </sec> <sec id="hed23196-sec-0004" sec-type="section"> <title>Conclusion</title> <p>These data highlight functional differences between tissue‐resident MSCs of the head and neck region, which may impact functional properties of these cells in response to trauma or infection. © 2012 Wiley Periodicals, Inc. <italic>Head Neck</italic>, <bold>35</bold>: 1621–1629, 2013</p> </sec> </abstract>