Analysis of brazilin and protosappanin B in sappan lignum by capillary zone electrophoresis with acid barrage stacking. Issue 24 (December 2013)

• Record Type: Journal Article
• Authors: Lu, Yuanqi; Bai, Hong; Kong, Chunyan; Zhong, Hao; Breadmore, Michael C.
• Is Part Of: Electrophoresis. Volume 34:Issue 24(2013:Dec.)
• Page Start: 3326
• Page End: 3332
• Publication Date: 2013-12
• Subjects: Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201300402
• Languages: English
• ISSNs: 0173-0835

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>A method was developed to determine brazilin and protosappanin B in natural products by CE after acid barrage stacking. The optimum conditions were as follows: a BGE of 20 mM sodium tetraborate (pH 9.2) containing 6% v/v of methanol, hydrodynamic injection (0.5 psi, 65 s) followed by hydrodynamic injection of 150 mM citric acid (pH 2.3; 0.5 psi, 22 s), and separated with +25 kV. Under these conditions, brazilin and protosappanin B were separated with a sample‐to‐sample time less than 13 min and detection limits of 0.28 μg/mL and 0.15 μg/mL, respectively. The applicability of the developed method was demonstrated by the detection of brazilin and protosappanin in methanol extract of sappan lignum.</p> </abstract>