Alternative visualization of SDS‐PAGE separated phosphoproteins by alizarin red S‐aluminum (III)‐appended complex. Issue 2 (12th December 2012)

• Record Type: Journal Article
• Authors: Wang, Xu; Hwang, Sun‐Young; Cong, Wei‐Tao; Jin, Li‐Tai; Choi, Jung‐Kap
• Is Part Of: Electrophoresis. Volume 34:Issue 2(2013:Jan.)
• Page Start: 235
• Page End: 243
• Publication Date: 2012-12-12
• Subjects: Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201200452
• Languages: English
• ISSNs: 0173-0835

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>A novel fluorescence detection system using a chemosensor for phosphoprotein in gel electrophoretic analysis has been developed. The system employed the alizarin red S‐aluminum (III)‐appended complex as a fluorescent staining dye to perform the convenient and selective detection of phosphorylated proteins and total proteins in SDS‐PAGE, respectively. Therefore, a full and selective map of proteins can be achieved in the same process without resorting to other compatible detection methods. As low as 62.5 ng of α‐ (seven or eight phosphates) and β‐casein (five phosphates), 125 ng of ovalbumin (two phosphates), and κ‐casein (one phosphate) can be detected in approximately 135 min, with the linear responses of rigorous quantitation of changes over a 125–4000 ng range. As a result, alizarin red S‐aluminum (III) stain may provide a new choice for selective, economic, and convenient visualization of phosphoproteins.</p> </abstract>