Electrochemical Estrogen Receptor α based Biosensor for Label‐Free Detection of Estradiol. Issue 7 (14th June 2013)

• Record Type: Journal Article
• Authors: Guo, Zhenzhong; Zine, Nadia; Balaguer, Patrick; Zhang, Aidong; Namour, Philippe; Lagarde, Florence; Jaffrezic‐Renault, Nicole
• Is Part Of: Electroanalysis. Volume 25:Issue 7(2013:Jul.)
• Page Start: 1765
• Page End: 1772
• Publication Date: 2013-06-14
• Subjects: Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201300163
• Languages: English
• ISSNs: 1040-0397

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>We describe a new electrochemical biosensor based on estrogen receptor α (ER‐α) for label‐free detection of 17β‐estradiol, a model of endocrine‐disrupting compounds. ER‐α is coupled onto the gold electrode through its 6‐His tag and NTA‐copper complex. After interaction of estradiol with ER‐α, the biosensor presents a well‐defined peak at +500 mV due to estradiol oxidation (E17 peak). The linear range of detection is from 1 fM to 1 nM and the detection limit is 1 fM. Good selectivity was obtained for interfering substances at nanomolar level, for concentration of E17 up to 0.1 pM. The E17 was detected in hospital effluents.</p> </abstract>