Investigation of Protein Binding With All Solid‐State Ion‐Selective Electrodes. Issue 8 (23rd July 2013)

• Record Type: Journal Article
• Authors: Prabhu, Alok; Bobacka, Johan; Ivaska, Ari; Levon, Kalle
• Is Part Of: Electroanalysis. Volume 25:Issue 8(2013:Aug.)
• Page Start: 1887
• Page End: 1894
• Publication Date: 2013-07-23
• Subjects: Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201300071
• Languages: English
• ISSNs: 1040-0397

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A potentiometric sensor for studying charge based adsorption of proteins was created using a single‐piece polyaniline‐PVC ion‐selective electrode (ISE). Three different ISEs, two for Na⁺ and one for Cl⁻ ion determination, were studied. The Na⁺‐ISEs consisted of a neutral calixarene‐based ionophore and one with a charged carrier dinonylnapthalenesulfonic acid (DNNSA) whereas for the Cl⁻ ISE, an anion exchanger tridodecylmethylammonium chloride (TDDMA⁺Cl⁻), was used. The Na⁺ ISE with DNNSA as the charged carrier was successfully able to discriminate the binding of two different proteins (bovine serum albumin and lysozyme) based on their intrinsic charge.</p> </abstract>