Oxygen Plasma Treatment of Platinized Ultramicroelectrodes Increases Sensitivity for Hydrogen Peroxide Detection on Mitochondria. Issue 3 (24th January 2013)

• Record Type: Journal Article
• Authors: Ben‐Amor, Salem; Devin, Anne; Rigoulet, Michel; Sojic, Neso; Arbault, Stéphane
• Is Part Of: Electroanalysis. Volume 25:Issue 3(2013:Mar.)
• Page Start: 656
• Page End: 663
• Publication Date: 2013-01-24
• Subjects: Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201200409
• Languages: English
• ISSNs: 1040-0397

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Hydrogen peroxide is a major bio‐active molecule produced by mitochondria during aerobic metabolism. In order to monitor that production, platinized ultramicroelectrodes with high surface‐area were used. Their sensitivity was significantly improved following an oxygen plasma treatment. Cyclic voltammetry and chronoamperometry analyses of ferricyanide reduction and hydrogen peroxide oxidation showed that ultramicroelectrodes' capacitive current was decreased 3‐fold, while faradaic currents remained constant. Then, plasma‐treated ultramicroelectrodes allowed the chronoamperometric detection of 100 nM H₂O₂ injections. This eventually enabled monitoring the mitochondria release of H₂O₂ following injection of substrates and a respiratory inhibitor; a flux of few hundreds picomoles H₂O₂/mg protein/min was detected.</p> </abstract>