Hybridization Detection of Osmium Tetroxide Bipyridine‐Labeled DNA and RNA on Heated Gold Wire Electrodes. Issue 2 (23rd December 2012)

• Record Type: Journal Article
• Authors: Jacobsen, Martin; Flechsig, Gerd‐Uwe
• Is Part Of: Electroanalysis. Volume 25:Issue 2(2013:Feb.)
• Page Start: 373
• Page End: 379
• Publication Date: 2012-12-23
• Subjects: Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201200460
• Languages: English
• ISSNs: 1040-0397

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>We report about hybridization detection of different nucleic acids on capture probe‐modified heated gold wire electrodes. We have compared three kinds of nucleic acid targets: DNA, uracil‐conjugated DNA, and RNA. All three sorts of nucleic acids targets could be labeled with osmium tetroxide bipyridine, hybridized with immobilized DNA capture probes and then detected by square‐wave voltammetry. Heating the gold electrode instead of the entire bulk hybridization solution leads to improved hybridization efficiency in most cases. The reason could be found in a thermal micro‐stirring effect around the heated wire electrode. Also selectivity was improved. Mismatches could be discriminated for DNA and uracil‐conjugated DNA targets. Mismatches in RNA strands, however, are more difficult to detect due to relatively stable secondary structures.</p> </abstract>