Long‐term, inducible gene loss‐of‐function in the chicken embryo. Issue 5 (26th March 2013)

• Record Type: Journal Article
• Authors: Serralbo, Olivier; Picard, Cyril A.; Marcelle, Christophe; Burt, David W.; Farlie, Peter G.
• Is Part Of: Genesis. Volume 51:Issue 5(2013:May)
• Page Start: 372
• Page End: 380
• Publication Date: 2013-03-26
• Subjects: Developmental genetics -- Periodicals; Genetics -- Periodicals; Developmental biology -- Periodicals; Embryology -- Periodicals; Genetic regulation -- Periodicals; 576.5
• DOI: 10.1002/dvg.22388
• Languages: English
• ISSNs: 1526-954X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The use of shRNAmir to down‐regulate the expression of genes of interest is a powerful tool for studying gene function during early chick development. However, because of the limitations of electroporation‐mediated transgenesis, the down‐regulation of genes expressed at late stages of development in specific tissues is difficult to perform. By combining electroporation of a doxycycline‐inducible, miR30‐based shRNA plasmid with the Tol2 genomic integration system, we are now able to down‐regulate the expression of any gene of interest at defined stage of chicken development. genesis 51:372–380. © 2013 Wiley Periodicals, Inc.</p> </abstract>