Etv2 rescues Flk1 mutant embryoid bodies. Issue 7 (30th May 2013)

• Record Type: Journal Article
• Authors: Rasmussen, Tara L.; Martin, Cindy M.; Walter, Camille A.; Shi, Xiaozhong; Perlingeiro, Rita; Koyano‐Nakagawa, Naoko; Garry, Daniel J.
• Is Part Of: Genesis. Volume 51:Issue 7(2013:Jul.)
• Page Start: 471
• Page End: 480
• Publication Date: 2013-05-30
• Subjects: Developmental genetics -- Periodicals; Genetics -- Periodicals; Developmental biology -- Periodicals; Embryology -- Periodicals; Genetic regulation -- Periodicals; 576.5
• DOI: 10.1002/dvg.22396
• Languages: English
• ISSNs: 1526-954X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Independent mouse knockouts of <italic>Etv2</italic> and <italic>Flk1</italic> are embryonic lethal and lack hematopoietic and endothelial lineages. We previously reported that Flk1 activates <italic>Etv2</italic> in the initiation of hematopoiesis and vasculogenesis. However, <italic>Flk1</italic> and its ligand <italic>VEGF</italic> are expressed throughout development, from E7.0 to adulthood, whereas <italic>Etv2</italic> is expressed only transiently during embryogenesis. These observations suggest a complex regulatory interaction between Flk1 and Etv2. To further examine the Flk1 and Etv2 regulatory interaction, we transduced <italic>Etv2</italic> and <italic>Flk1</italic> mutant ES cells with viral integrants that inducibly overexpress Flk1 or Etv2. We demonstrated that forced expression of Etv2 rescued the hematopoietic and endothelial potential of differentiating <italic>Flk1</italic> and <italic>Etv2</italic> mutant cells. We further discovered that forced expression of Flk1 can rescue that of the <italic>Flk1</italic>, but not <italic>Etv2</italic> mutant cells. Therefore, we conclude that the requirement for Flk1 can be bypassed by expressing Etv2, supporting the notion that disruption of Etv2 expression is responsible for the early phenotypes of the <italic>Etv2</italic> and <italic>Flk1</italic> mutant embryos. genesis 51:471–480.© 2013 Wiley Periodicals, Inc.</p> </abstract>