A Phox2b:FLPo transgenic mouse line suitable for intersectional genetics. Issue 7 (22nd May 2013)

• Record Type: Journal Article
• Authors: Hirsch, Marie‐Rose; d'Autréaux, Fabien; Dymecki, Susan M.; Brunet, Jean‐François; Goridis, Christo
• Is Part Of: Genesis. Volume 51:Issue 7(2013:Jul.)
• Page Start: 506
• Page End: 514
• Publication Date: 2013-05-22
• Subjects: Developmental genetics -- Periodicals; Genetics -- Periodicals; Developmental biology -- Periodicals; Embryology -- Periodicals; Genetic regulation -- Periodicals; 576.5
• DOI: 10.1002/dvg.22393
• Languages: English
• ISSNs: 1526-954X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Phox2b is a transcription factor expressed in the central and peripheral neurons that control cardiovascular, respiratory, and digestive functions and essential for their development. Several populations known or suspected to regulate visceral functions express Phox2b in the developing hindbrain. Extensive cell migration and lack of suitable markers have greatly hampered studying their development. Reasoning that intersectional fate mapping may help to overcome these impediments, we have generated a BAC transgenic mouse line, <italic>P2b::FLPo</italic>, which expresses codon‐optimized FLP recombinase in <italic>Phox2b</italic> expressing cells. By partnering the <italic>P2b::FLPo</italic> with the FLP‐responsive RC::Fela allele, we show that FLP recombination switches on lineage tracers in the cells that express or have expressed Phox2b, permanently marking them for study across development. Taking advantage of the dual‐recombinase feature of RC::Fela, we further show that the <italic>P2b::FLPo</italic> transgene can be partnered with <italic>Lbx1<sup>Cre</sup></italic> as Cre driver to generate triple transgenics in which neurons having a history of both <italic>Phox2b</italic> and <italic>Lbx1</italic> expression are specifically labeled. Hence, the <italic>P2b::FLPo</italic> line when partnered with a suitable Cre driver provides a tool for tracking and accessing genetically subsets of Phox2b‐expressing neuronal populations, which has not been possible by Cre‐mediated recombination alone. genesis 51:506–514. © 2013 Wiley Periodicals, Inc.</p> </abstract>