Generation and characterization of a tamoxifen‐inducible EomesCreER mouse line. Issue 10 (30th August 2013)

• Record Type: Journal Article
• Authors: Pimeisl, Inga‐Marie; Tanriver, Yakup; Daza, Ray A.; Vauti, Franz; Hevner, Robert F.; Arnold, Hans‐Henning; Arnold, Sebastian J.
• Is Part Of: Genesis. Volume 51:Issue 10(2013:Oct.)
• Page Start: 725
• Page End: 733
• Publication Date: 2013-08-30
• Subjects: Developmental genetics -- Periodicals; Genetics -- Periodicals; Developmental biology -- Periodicals; Embryology -- Periodicals; Genetic regulation -- Periodicals; 576.5
• DOI: 10.1002/dvg.22417
• Languages: English
• ISSNs: 1526-954X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Transgenic mouse lines expressing inducible forms of Cre‐recombinase in a tissue‐specific manner are powerful genetic tools for studying aspects of development and various processes in the adult. The T‐box transcription factor eomesodermin (Eomes) plays critical roles for maintenance and differentiation of different pools of stem and progenitor cells from early embryonic stages to adulthood. These include trophoblast stem cells, epiblast cells during the generation of the primary germ layers, neurogenic intermediate progenitor cells in embryonic and adult cortical neurogenesis, and maturing natural killer and T cells. Here, we report on the generation and analysis of an Eomes<sup>CreER</sup>‐targeted allele by placing the tamoxifen‐activatable <italic>Cre‐recombinase</italic> (<italic>CreER</italic>) under the control of the <italic>Eomes</italic> genomic locus. We demonstrate that <italic>CreER</italic> expression recapitulates endogenous <italic>Eomes</italic> transcription within different progenitor cell populations. Tamoxifen administration specifically labels <italic>Eomes</italic>‐expressing cells and their progeny as demonstrated by crossing Eomes<sup>CreER</sup> animals to different Cre‐inducible reporter strains. In summary, this novel Eomes<sup>CreER</sup> allele can be used as elegant genetic tool that allows to follow the fate of Eomes‐positive cells and to genetically manipulate them in a temporal specific manner. genesis 51:725–733. © 2013 Wiley Periodicals, Inc.</p> </abstract>