503unc, a small and muscle‐specific zebrafish promoter. Issue 6 (26th March 2013)

• Record Type: Journal Article
• Authors: Berger, Joachim; Currie, Peter D.
• Is Part Of: Genesis. Volume 51:Issue 6(2013:Jun.)
• Page Start: 443
• Page End: 447
• Publication Date: 2013-03-26
• Subjects: Developmental genetics -- Periodicals; Genetics -- Periodicals; Developmental biology -- Periodicals; Embryology -- Periodicals; Genetic regulation -- Periodicals; 576.5
• DOI: 10.1002/dvg.22385
• Languages: English
• ISSNs: 1526-954X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The muscle‐specific UNC‐45b assists in the folding of sarcomeric myosin. Analysis of the zebrafish <italic>unc‐45b</italic> upstream region revealed that <italic>unc‐45b</italic> promoter fragments reliably drive GFP expression after germline transmission. The muscle‐specific 503‐bp minimal promoter <italic>503unc</italic> was identified to drive gene expression in the zebrafish musculature. In transgenic <italic>Tg(−503unc:GFP)</italic> zebrafish, GFP fluorescence was detected in the adaxial cells, their slow fiber descendants, and the fast muscle. At later stages, robust GFP fluorescence is eminent in the cardiac, cranial, fin, and trunk muscle, thereby recapitulating the <italic>unc‐45b</italic> expression pattern. We propose that the <italic>503unc</italic> promoter is a small and muscle‐specific promoter that drives robust gene expression throughout the zebrafish musculature, making it a valuable tool for the exploration of zebrafish muscle. genesis 51:443–447. © 2013 Wiley Periodicals, Inc.</p> </abstract>