EChickAtlas: An introduction to the database. Issue 5 (30th March 2013)
- Record Type:
- Journal Article
- Title:
- EChickAtlas: An introduction to the database. Issue 5 (30th March 2013)
- Main Title:
- EChickAtlas: An introduction to the database
- Authors:
- Wong, Frances
Welten, Monique C.M.
Anderson, Claire
Bain, Andrew A.
Liu, Jiahui
Wicks, Michael N.
Pavlovska, Gordana
Davey, Megan G.
Murphy, Paula
Davidson, Duncan
Tickle, Cheryll A.
Stern, Claudio D.
Baldock, Richard A.
Burt, David W.
Burt, David W.
Farlie, Peter G. - Abstract:
- <abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The precise control of gene expression is critical in embryonic development. Quantitative assays, such as microarrays and RNA sequencing, provide gene expression levels for a large number of genes, but do not contain spatial information. In contrast, <italic>in situ</italic> methods, such as <italic>in situ</italic> hybridization and immunohistochemistry, provide spatial resolution, but poor quantification and can only reveal the expression of one, or very few genes at a time. Furthermore, the usual methods of documenting the results, by photographing whole mounts or sections, makes it very difficult to assess the three‐dimensional (3D) relationships between expressing and nonexpressing cells. Optical projection tomography (OPT) can capture the full 3D expression pattern in a whole embryo at a reasonable level of resolution and at moderately high throughput. A large database containing spatio‐temporal patterns of expression for the mouse (e‐Mouse Atlas Project, EMAP, www.emouseatlas.org) has been created, incorporating 3D information. Like the mouse, the chick is an important model in developmental biology and translational studies. To facilitate comparisons between these important model organisms, we have created a 3D anatomical atlas, accompanied by an anatomical ontology of the chick embryo and a database of gene expression patterns during chick development. This database is publicly<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>The precise control of gene expression is critical in embryonic development. Quantitative assays, such as microarrays and RNA sequencing, provide gene expression levels for a large number of genes, but do not contain spatial information. In contrast, <italic>in situ</italic> methods, such as <italic>in situ</italic> hybridization and immunohistochemistry, provide spatial resolution, but poor quantification and can only reveal the expression of one, or very few genes at a time. Furthermore, the usual methods of documenting the results, by photographing whole mounts or sections, makes it very difficult to assess the three‐dimensional (3D) relationships between expressing and nonexpressing cells. Optical projection tomography (OPT) can capture the full 3D expression pattern in a whole embryo at a reasonable level of resolution and at moderately high throughput. A large database containing spatio‐temporal patterns of expression for the mouse (e‐Mouse Atlas Project, EMAP, www.emouseatlas.org) has been created, incorporating 3D information. Like the mouse, the chick is an important model in developmental biology and translational studies. To facilitate comparisons between these important model organisms, we have created a 3D anatomical atlas, accompanied by an anatomical ontology of the chick embryo and a database of gene expression patterns during chick development. This database is publicly available (www.echickatlas.org). genesis 51:365–371. © 2013 Wiley Periodicals, Inc.</p> </abstract> … (more)
- Is Part Of:
- Genesis. Volume 51:Issue 5(2013:May)
- Journal:
- Genesis
- Issue:
- Volume 51:Issue 5(2013:May)
- Issue Display:
- Volume 51, Issue 5 (2013)
- Year:
- 2013
- Volume:
- 51
- Issue:
- 5
- Issue Sort Value:
- 2013-0051-0005-0000
- Page Start:
- 365
- Page End:
- 371
- Publication Date:
- 2013-03-30
- Subjects:
- Developmental genetics -- Periodicals
Genetics -- Periodicals
Developmental biology -- Periodicals
Embryology -- Periodicals
Genetic regulation -- Periodicals
576.5 - Journal URLs:
- http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1526-968X ↗
http://onlinelibrary.wiley.com/ ↗ - DOI:
- 10.1002/dvg.22374 ↗
- Languages:
- English
- ISSNs:
- 1526-954X
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 4111.807500
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 3040.xml