Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development. Issue 8 (18th June 2013)

• Record Type: Journal Article
• Authors: Filipek‐Górniok, Beata; Holmborn, Katarina; Haitina, Tatjana; Habicher, Judith; Oliveira, Marta Bastos; Hellgren, Charlotte; Eriksson, Inger; Kjellén, Lena; Kreuger, Johan; Ledin, Johan
• Is Part Of: Developmental dynamics. Volume 242:Issue 8(2013:Aug.)
• Page Start: 964
• Page End: 975
• Publication Date: 2013-06-18
• Subjects: Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.23981
• Languages: English
• ISSNs: 1058-8388

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <underline>Background:</underline> Chondroitin/dermatan sulfate (CS/DS) proteoglycans present in the extracellular matrix have important structural and regulatory functions. <underline>Results:</underline> Six human genes have previously been shown to catalyze CS/DS polymerization. Here we show that one of these genes, <italic>chpf</italic>, is represented by two copies in the zebrafish genome, <italic>chpfa</italic> and <italic>chpfb</italic>, while the other five human CS/DS glycosyltransferases <italic>csgalnact1, csgalnact2, chpf2, chsy1, </italic> and <italic>chsy3</italic> all have single zebrafish orthologues. The putative zebrafish CS/DS glycosyltransferases are spatially and temporally expressed. Interestingly, overlapping expression of multiple glycosyltransferases coincides with high CS/DS deposition. Finally, whereas the relative levels of the related polysaccharide HS reach steady‐state at around 2 days post fertilization, there is a continued relative increase of the CS amounts per larvae during the first 6 days of development, matching the increased cartilage formation. <underline>Conclusions:</underline> There are 7 CS/DS glycosyltransferases in zebrafish, which, based on homology, can be divided into the CSGALNACT, CHSY, and CHPF families. The overlap between intense CS/DS production and the expression of multiple CS/DS glycosyltransferases suggests that efficient CS/DS biosynthesis requires a combination of several glycosyltransferases. <italic>Developmental Dynamics 242:964–975, 2013</italic>. © 2013 Wiley Periodicals, Inc.</p> </abstract>