Prediction of outcome in patients with low‐grade squamous intraepithelial lesions by fluorescence in situ hybridization analysis of human papillomavirus, TERC, and MYC. Issue 8 (13th February 2013)

• Record Type: Journal Article
• Authors: Obermann, Ellen C.; Savic Prince, Spasenija; Barascud, Audrey; Grilli, Bruno; Herzog, Michelle; Kaup, Daniela; Cathomas, Gieri; Frey Tirri, Brigitte; Zlobec, Inti; Wight, Edward; Bubendorf, Lukas
• Is Part Of: Cancer cytopathology. Volume 121:Issue 8(2013:Aug.)
• Page Start: 423
• Page End: 431
• Publication Date: 2013-02-13
• Subjects: Cancer -- Cytopathology -- Periodicals; Pathology, Cellular -- Periodicals; Cytology -- Technique -- Periodicals; 611.01815
• DOI: 10.1002/cncy.21280
• Languages: English
• ISSNs: 1934-662X

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="cncy21280-sec-0001" sec-type="section"> <title>BACKGROUND</title> <p>Cytology is an excellent method with which to diagnose preinvasive lesions of the uterine cervix, but it suffers from limited specificity for clinically significant lesions. Supplementary methods might predict the natural course of the detected lesions. The objective of the current study was to test whether a multicolor fluorescence in situ hybridization (FISH) assay might help to stratify abnormal results of Papanicolaou tests.</p> </sec> <sec id="cncy21280-sec-0002" sec-type="section"> <title>METHODS</title> <p>A total of 219 liquid‐based cytology specimens of low‐grade squamous intraepithelial lesions (LSIL), 49 atypical squamous cells of undetermined significance (ASCUS) specimens, 52 high‐grade squamous intraepithelial lesion (HSIL) specimens, and 50 normal samples were assessed by FISH with probes for the human papillomavirus (HPV), <italic>MYC, </italic> and telomerase RNA component (<italic>TERC</italic>). Subtyping of HPV by polymerase chain reaction (PCR) was performed in a subset of cases (n=206).</p> </sec> <sec id="cncy21280-sec-0003" sec-type="section"> <title>RESULTS</title> <p>There was a significant correlation found between HPV detection by FISH and PCR (<italic>P</italic><.0001). In patients with LSILs, the presence of HPV detected by FISH was significantly associated with disease progression (<italic>P</italic><.0001). An increased <italic>MYC</italic> and/or <italic>TERC</italic> gene copy number (>2 signals in>10% of cells) prevailed in 43% of ASCUS specimens and was more frequent in HSIL (85%) than in LSIL (33%) (HSIL vs LSIL: <italic>P</italic><.0001). Increased <italic>TERC</italic> gene copy number was significantly correlated with progression of LSIL (<italic>P</italic><.01; odds ratio, 7.44; area under the receiver operating characteristic curve, 0.73; positive predictive value, 0.30; negative predictive value, 0.94)</p> </sec> <sec id="cncy21280-sec-0004" sec-type="section"> <title>CONCLUSIONS</title> <p>The detection of HPV by FISH analysis is feasible in liquid‐based cytology and is significantly correlated with HPV analysis by PCR. The analysis of <italic>TERC</italic> gene copy number may be useful for risk stratification in patients with LSIL. <bold><italic>Cancer (Cancer Cytopathol)</italic> 2013;121:423–31.</bold> © <italic>2013 American Cancer Society</italic>.</p> </sec> </abstract>