Modulation of Golgi‐associated microtubule nucleation throughout the cell cycle 1. Issue 1 (17th October 2012)

• Record Type: Journal Article
• Authors: Maia, Ana Rita Ramada; Zhu, Xiaodong; Miller, Paul; Gu, Guoqiang; Maiato, Helder; Kaverina, Irina
• Is Part Of: Cytoskeleton. Volume 70:Issue 1(2013:Jan.)
• Page Start: 32
• Page End: 43
• Publication Date: 2012-10-17
• Subjects: Cytoskeleton -- Periodicals; 571.65405
• DOI: 10.1002/cm.21079
• Languages: English
• ISSNs: 1949-3584

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A microtubule (MT) subpopulation that emanates from Golgi membrane has been recently shown to comprise a significant part of MT network in interphase cells. In this study, we address whether Golgi membrane, which is being extensively remodeled throughout the cell cycle, retains its ability to nucleate MTs at diverse cell cycle stages. Live cell imaging and immunofluorescence microscopy reveals that Golgi‐derived MTs form at multiple stages of the cell cycle, including G₁, G₂, and distinct phases of mitosis. However, the capacity of Golgi to nucleate MTs in mitosis is strongly down‐regulated as compared with interphase, indicating that this property is cell cycle regulated. We demonstrate that Golgi‐derived MTs are indispensable for efficient Golgi assembly in telophase, and speculate that these noncentrosomal MTs may hold specific functions at other cell cycle stages. © 2012 Wiley Periodicals, Inc</p> </abstract>