Direct Electrochemistry of Hemoglobin on Vertically Aligned Carbon Hybrid TiO2 Nanotubes and Its Highly Sensitive Biosensor Performance. Issue 2 (11th December 2012)

• Record Type: Journal Article
• Authors: Liu, Meichuan; Zhao, Guohua; Tang, Yiting; Shi, Huijie; Yang, Nianjun
• Is Part Of: Chinese journal of chemistry. Volume 31:Issue 2(2013:Feb.)
• Page Start: 215
• Page End: 220
• Publication Date: 2012-12-11
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1002/cjoc.201200883
• Languages: English
• ISSNs: 1001-604X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>The present work is focused on developing a novel biomaterial platform to achieve enhanced direct electron transfer (DET) of hemoprotein and higher biosensor performance on vertically aligned carbon hybrid TiO<sub>2</sub> nanotubes (C‐TiO<sub>2</sub> NTs). Using a simple surfactant‐assisted method, controllable hybridization of TiO<sub>2</sub> NTs with conductive amorphous carbon species is realized. The obtained C‐TiO<sub>2</sub> NTs is ingeniously chosen to serve as an ideal "vessel" for protein immobilization and biosensor applications. Results show that the appropriate hybridization of C into TiO<sub>2</sub> NTs leads to a much better conductivity of TiO<sub>2</sub> NTs without destroying their preponderant tubular structures or damaging their excellent biocompatibility and hydrophilicity. When used in loading proteins, the C‐TiO<sub>2</sub> NTs can be used as a super vessel for rapid and substantive immobilization of hemoglobin (Hb), with a large surface electroactive Hb coverage (<italic><bold>Γ</bold></italic>∗︁) of 3.3×10<sup>−9</sup> mol·cm<sup>−2</sup>. Enhanced DET of Hb is commendably observed on the constructed Hb/C‐TiO<sub>2</sub> NTs biosensor with a couple of well‐defined redox peaks in a fast electron transfer process. The biosensor further exhibits fast response, high sensitivity and stability for the amperometric biosensing of H<sub>2</sub>O<sub>2</sub> with the detection limit as low as 3.1×10<sup>−8</sup> mol/L.</p> </abstract>