Cultivation of Cells and Microorganisms in Wave‐Mixed Disposable Bag Bioreactors at Different Scales. Issue 1 (13th December 2012)

• Record Type: Journal Article
• Authors: Junne, Stefan; Solymosi, Thomas; Oosterhuis, Nico; Neubauer, Peter; Eibl, Regine; Eibl, Dieter
• Is Part Of: Chemie Ingenieur Technik. Volume 85:Issue 1/2(2013)
• Page Start: 57
• Page End: 66
• Publication Date: 2012-12-13
• Subjects: Chemical engineering -- Patents -- Periodicals; Chemical engineering -- Periodicals; Chemical industry -- Periodicals; Chemistry, Technical -- Periodicals; 660.05
• DOI: 10.1002/cite.201200149
• Languages: English
• ISSNs: 0009-286X

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Disposable bioreactors have gained an increasing importance in recent years in the pharmaceutical production. Wave‐mixed reactors were among the first systems which were applied. In contrast to stirred tank reactors, wave‐mixed bioreactors are characterized by low shear forces while the gas exchange is realized by the large gas‐liquid interface. Oxygen transfer rates obtained are often in a range between 50 and 300 h^–1. By applying nutrient‐limiting fed‐batch cultivations, in which the oxygen consumption of a culture is controlled, bacteria can be also cultivated in wave‐mixed reactors. This article describes the successful scale‐up of an <italic>Escherichia coli</italic> fed‐batch cultivation from the 12‐L to the 120‐L scale using a disposable bioreactor, in which a final biomass concentration of 45 g L^–1 was obtained.</p> </abstract>