Enzymatic Fragment Condensation of Side Chain‐Protected Peptides using Subtilisin A in Anhydrous Organic Solvents: A General Strategy for Industrial Peptide Synthesis. Issue 2 (4th January 2013)

• Record Type: Journal Article
• Authors: Nuijens, Timo; Schepers, Annette H. M.; Cusan, Claudia; Kruijtzer, John A. W.; Rijkers, Dirk T. S.; Liskamp, Rob M. J.; Quaedflieg, Peter J. L. M.
• Is Part Of: Advanced synthesis & catalysis. Volume 355:Issue 2/3(2013)
• Page Start: 287
• Page End: 293
• Publication Date: 2013-01-04
• Subjects: Catalysis -- Periodicals; Organic compounds -- Synthesis -- Periodicals; Chemistry -- Periodicals; Chemistry, Technical -- Periodicals; Chemistry -- Periodicals; Catalysis -- Periodicals; Technology, Pharmaceutical -- Periodicals; 547.2
• DOI: 10.1002/adsc.201200694
• Languages: English
• ISSNs: 1615-4150

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Herein, the enzymatic condensation of side chain‐protected peptide fragments using subtilisin A in anhydrous organic solvents is described. A screening with dipeptide Cbz‐Val‐Xxx carboxamidomethyl esters with H‐Xxx‐Val‐NH₂ nucleophiles was performed, wherein Xxx stands for every (side chain‐protected) amino acid residue. Finally, it was demonstrated that it is feasible to enzymatically condense larger peptide fragments (up to the 10‐mer level) bearing multiple side chain‐protecting groups with very high conversion.</p> </abstract>