Artificial Multi‐Enzyme Networks for the Asymmetric Amination of sec‐Alcohols. Issue 12 (22nd January 2013)

• Record Type: Journal Article
• Authors: Tauber, Katharina; Fuchs, Michael; Sattler, Johann H.; Pitzer, Julia; Pressnitz, Desiree; Koszelewski, Dominik; Faber, Kurt; Pfeffer, Jan; Haas, Thomas; Kroutil, Wolfgang
• Is Part Of: Chemistry. Volume 19:Issue 12(2013)
• Page Start: 4030
• Page End: 4035
• Publication Date: 2013-01-22
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201202666
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Various artificial network designs that involve biocatalysts were tested for the asymmetric amination of <italic>sec</italic>‐alcohols to the corresponding α‐chiral primary amines. The artificial systems tested involved three to five redox enzymes and were exemplary of a range of different <italic>sec</italic>‐alcohol substrates. Alcohols were oxidised to the corresponding ketone by an alcohol dehydrogenase. The ketones were subsequently aminated by employing a ω‐transaminase. Of special interest were redox‐neutral designs in which the hydride abstracted in the oxidation step was reused in the amination step of the cascade. Under optimised conditions up to 91 % conversion of an alcohol to the amine was achieved.</p> </abstract>