Rapid and Specific Post‐Synthesis Modification of DNA through a Biocompatible Condensation of 1, 2‐Aminothiols with 2‐Cyanobenzothiazole. Issue 12 (28th February 2013)

• Record Type: Journal Article
• Authors: Cheng, Yunfeng; Peng, Hanjing; Chen, Weixuan; Ni, Nanting; Ke, Bowen; Dai, Chaofeng; Wang, Binghe
• Is Part Of: Chemistry. Volume 19:Issue 12(2013)
• Page Start: 4036
• Page End: 4042
• Publication Date: 2013-02-28
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1002/chem.201201677
• Languages: English
• ISSNs: 0947-6539

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Post‐synthesis modification of DNA is an important way of functionalizing DNA molecules. Herein, we describe a method that first enzymatically incorporates a cyanobenzothiazole (CBT)‐modified thymidine. The side‐chain handle CBT can undergo a rapid and site‐specific cyclization reaction with 1, 2‐aminothiols to afford DNA functionalization in aqueous solution. Another key advantage of this method is the formation of a single stereo/regioisomer in the process, which allows for precise control of DNA modification to yield a single component for aptamer selection work and other applications.</p> </abstract>