Profiling Glycosyltransferase Activities by Tritium Imaging of Glycan Microarrays. Issue 7 (8th April 2013)

• Record Type: Journal Article
• Authors: Serna, Sonia; Hokke, Cornelis H.; Weissenborn, Martin; Flitsch, Sabine; Martin‐Lomas, Manuel; Reichardt, Niels‐Christian
• Is Part Of: Chembiochem. Volume 14:Issue 7(2013)
• Page Start: 862
• Page End: 869
• Publication Date: 2013-04-08
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300051
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>High‐throughput microarray technology</bold> has been combined with ultrasensitive and high‐resolution tritium autoradiography to create a new platform for the quantitative detection of glycosyltransferase activity on glycan arrays. In addition, we show full compatibility with the use of fluorescently labeled lectins to help with the stereochemical assignment of newly formed glycoside linkages.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1zt4q6w8" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>