A Click‐and‐Release Pyrrolysine Analogue. Issue 7 (15th April 2013)

• Record Type: Journal Article
• Authors: Lee, Marianne M.; Fekner, Tomasz; Tang, Tsz‐Ho; Wang, Lin; Chan, Aurora Ho‐Yin; Hsu, Pang‐Hung; Au, Shannon W.; Chan, Michael K.
• Is Part Of: Chembiochem. Volume 14:Issue 7(2013)
• Page Start: 805
• Page End: 808
• Publication Date: 2013-04-15
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300124
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>What's the catch?</bold> A pyrrolysine analogue bearing a terminal alkyne and an ester functionality can be incorporated into recombinant proteins and render them amenable to capture by the click reaction and subsequent release through ester hydrolysis. The utility of this pyrrolysine‐inspired technology is demonstrated for the identification of SUMOylation sites.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1zt4q5pf" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>