Unraveling the Interaction between the LPS O‐Antigen of Burkholderia anthina and the 5D8 Monoclonal Antibody by Using a Multidisciplinary Chemical Approach, with Synthesis, NMR, and Molecular Modeling Methods. Issue 12 (22nd July 2013)

• Record Type: Journal Article
• Authors: Marchetti, Roberta; Canales, Angeles; Lanzetta, Rosa; Nilsson, Inga; Vogel, Christian; Reed, Dana E.; AuCoin, David P.; Jiménez‐Barbero, Jesús; Molinaro, Antonio; Silipo, Alba
• Is Part Of: Chembiochem. Volume 14:Issue 12(2013)
• Page Start: 1485
• Page End: 1493
• Publication Date: 2013-07-22
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201300225
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>The interaction between the O‐chain from the lipopolysaccharide from <italic>Burkholderia anthina</italic> and a lipopolysaccharide‐specific monoclonal antibody (5D8) has been studied at high resolution by NMR spectroscopy. In particular, the 5D8‐bound epitope of the saccharide entity has been unraveled by a combination of saturation transfer difference (STD) and transferred NOESY (tr‐NOESY) experiments performed on the 5D8/polysaccharide complex. To dissect the fine details of the molecular recognition events, further experiments with simpler carbohydrate ligands were carried out. Thus, experiments were also performed with ad hoc synthesized trisaccharide and hexasaccharide O‐antigen repeating units. By using this multidisciplinary approach (chemical synthesis, NMR spectroscopy and molecular dynamics simulation), determination of the binding epitope and the contribution to the binding of the sugar units composing the O‐chain have been determined.</p> </abstract>