Photobleaching Lifetimes of Cyanine Fluorophores Used for Single‐Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems. Issue 9 (3rd June 2013) - Cooper, David
Abstract Lengthening smFRET lifetimes: We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single‐molecule environment. We found that the glucose/glucose oxygen‐scavenging solution in combination with redox‐based photostabilization solutions yielded the longest average photobleaching lifetimes.