Cover Picture: Effect of Ganglioside GM3 Synthase Gene Knockout on the Glycoprotein N‐Glycan Profile of Mouse Embryonic Fibroblast (ChemBioChem 1/2013). Issue 1 (23rd December 2012)

• Record Type: Journal Article
• Authors: Nagahori, Noriko; Yamashita, Tadashi; Amano, Maho; Nishimura, Shin‐Ichiro
• Is Part Of: Chembiochem. Volume 14:Issue 1(2013)
• Page Start: 1
• Page End: 1
• Publication Date: 2012-12-23
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201290078
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="graphical" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <bold>The cover picture shows</bold> that gene knockout of the enzymes responsible for glycosphingolipid (GSL) synthesis significantly influences the biosynthesis of glycoprotein <italic>N</italic>‐glycans. On <ext-link ext-link-type="doi" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">p. 73 ff., </ext-link> S.‐I. Nishimura et al. describe how they have established a general and comprehensive glycomics protocol of cellular GLSs and <italic>N</italic>‐glycans of glycoproteins. To test the feasibility of a glycoblotting‐based protocol, whole glycans released both from GLSs and glycoproteins were profiled concurrently by using the GM3 synthase‐deficient mouse embryonic fibroblast GM3(−/−). GM3(−/−) cells did not synthesize GM3 or any downstream product of GM3 synthase. Instead, expression levels of o‐series gangliosides involving GM1b and GD1‐α were increased dramatically whereas a‐/b‐series gangliosides were predominantly detected in wild‐type cells. It was also discovered that glycoprotein N‐glycan profiles of GM3(−/−) cells are significantly different from those of WT cells, though GM3 synthase is responsible only for GSL synthesis and is not associated with glycoprotein <italic>N</italic>‐glycan biosynthesis.<boxed-text content-type="graphic" position="anchor" orientation="portrait"><graphic position="anchor" mimetype="image" xlink:href="ark:/27927/pgg1p96pjcb" orientation="portrait" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink" /></boxed-text></p> </abstract>