Capture of Uropathogenic E. coli by Using Synthetic Glycan Ligands Specific for the Pap‐Pilus. Issue 2 (10th January 2013)

• Record Type: Journal Article
• Authors: Yosief, Hailemichael O.; Weiss, Alison A.; Iyer, Suri S.
• Is Part Of: Chembiochem. Volume 14:Issue 2(2013)
• Page Start: 251
• Page End: 259
• Publication Date: 2013-01-10
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201200582
• Languages: English
• ISSNs: 1439-4227

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Biotinylated mono‐ and biantennary di‐/trisaccharides were synthesized to evaluate their ability to capture <italic>E. coli</italic> strains that express pilus types with different receptor specificities. The synthesized biotinylated di‐/trisaccharides contain Galα(1→4)Gal, Galα(1→4)GalNHAc, GalNHAcα(1→4)Gal, Galα(1→4)Galβ(1→4)Glc and GalNHAcα(1→4)Galβ(1→4)Glc as carbohydrate epitopes. These biotinylated oligosaccharides were immobilized on streptavidin‐coated magnetic beads, and incubated with different strains of live <italic>E. coli</italic>. Capturing ability was assessed by using a luciferase assay that detects bacterial ATP. The trisaccharides containing Galα(1→4)Galβ(1→4)Glc and the disaccharides containing Galα(1→4)Gal as the epitopes exhibited strong capturing ability for uropathogenic <italic>E. coli</italic> strains with the <italic>pap</italic> pilus genotype, including CFT073, J96 and J96 pilE. The same ligands failed to capture <italic>E. coli</italic> strains with <italic>fim</italic>, <italic>prs</italic>, or <italic>foc</italic> genotypes. Uropathogenic CFT073 was also captured moderately by biantennary disaccharides containing a GalNHAc moiety at the reducing end; however, other saccharides containing GalNHAc at the nonreducing end did not capture the CFT073 strain. These synthetic glycoconjugates could potentially be adapted as rapid diagnostic agents to differentiate between different <italic>E. coli</italic> pathovars.</p> </abstract>