Bactericidal properties of the antimicrobial peptide Ib‐AMP4 from Impatiens balsamina produced as a recombinant fusion‐protein in Escherichia coli. Issue 10 (21st June 2013)

• Record Type: Journal Article
• Authors: Fan, Xiaobo; Schäfer, Holger; Reichling, Jürgen; Wink, Michael
• Is Part Of: Biotechnology journal. Volume 8:Issue 10(2013:Oct.)
• Page Start: 1213
• Page End: 1220
• Publication Date: 2013-06-21
• Subjects: Biotechnology -- Periodicals; 660.605
• DOI: 10.1002/biot.201300121
• Languages: English
• ISSNs: 1860-6768

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Antimicrobial peptides (AMPs) represent a novel class of powerful natural antimicrobial agents. As AMPs are bactericidal, production of AMPs in recombinant bacteria is far from trivial. We report the production of <italic>Impatiens balsamina</italic> antimicrobial peptide 4 (Ib‐AMP4, originally isolated from <italic>Impatiens balsamina</italic>) in <italic>Escherichia coli</italic> as a fusion protein and investigate Ib‐AMP4's antimicrobial effects on human pathogens. A plasmid vector <italic>pET32a‐Trx‐Ib‐AMP4</italic> was constructed and transferred into <italic>E. coli</italic>. After induction, a soluble fusion protein was expressed successfully. The Ib‐AMP4 peptide was obtained with a purity of over 90% after nickel affinity chromatography, ultrafiltration, enterokinase cleavage and sephadex size exclusion chromatography. For maximum activity, Ib‐AMP4, which possesses two disulfide bonds, required activation with 5 μg/mL H<sub>2</sub>O<sub>2</sub>. Antimicrobial assays showed that Ib‐AMP4 could efficiently target clinical multiresistant isolates including methicillin‐resistant <italic>Staphylococcus aureus</italic> and extended‐spectrum β‐lactamase‐producing <italic>E. coli</italic>. Time kill experiments revealed that Ib‐AMP4 is bactericidal within 10 min after application. Haemolysis and cytotoxicity assays implied selectivity towards bacteria, an important prerequisite for clinical applications. Ib‐AMP4 might be an interesting candidate for clinical studies involving patients with septicemia or for coating clinical devices, such as catheters. The method described here may be applicable for expression and purification of other AMPs with multiple disulfide bridges.</p> </abstract>