Real‐Time Fluorescence Turn‐On Detection of Alkaline Phosphatase Activity with a Novel Perylene Probe. Issue 1 (9th November 2012)

• Record Type: Journal Article
• Authors: Chen, Jian; Jiao, Huping; Li, Wenying; Liao, Dongli; Zhou, Huipeng; Yu, Cong
• Is Part Of: Chemistry, an Asian journal. Volume 8:Issue 1(2013:Jan.)
• Page Start: 276
• Page End: 281
• Publication Date: 2012-11-09
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.201200880
• Languages: English
• ISSNs: 1861-4728

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A tetracationic perylene probe (probe <bold>1</bold>) was designed and synthesized. Probe <bold>1</bold> was used for the real‐time fluorescence turn‐on assay of alkaline phosphatase (ALP) activity and inhibitor screening. Probe <bold>1</bold> monomer fluorescence could be very efficiently quenched by ATP through the formation of an ATP/probe <bold>1</bold> complex. ALP triggered the degradation of ATP, the breakdown of the ATP/probe <bold>1</bold> complex, and the recovery of the probe <bold>1</bold> monomer fluorescence. In the presence of an ALP inhibitor, a decrease in fluorescence recovery was observed.</p> </abstract>