Porous and spherical hydroxyapatite microcomposites for immunoglobulin G adsorption. Issue 4 (3rd June 2013)

• Record Type: Journal Article
• Authors: Akkaya, Birnur
• Is Part Of: Journal of applied polymer science. Volume 130:Issue 4(2013:Nov. 15)
• Page Start: 2764
• Page End: 2771
• Publication Date: 2013-06-03
• Subjects: Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.39341
• Languages: English
• ISSNs: 0021-8995

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>A novel porous and spherical hydroxyapatite (HA) microcomposite with an average diameter of 50–100 μm was produced by suspension polymerization for the adsorption of human immunoglobulin G (IgG). The specific surface area of the pure HA and HA microcomposite were found to be 80.25 and 182.53 m²/g, respectively. Characterizations were performed with X‐ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analysis, and scanning electron microscopy analysis. The swelling ratio of the spherical HA microcomposites was 170%. The maximum adsorption of IgG from aqueous solution was observed at pH 7.0 (5 m<italic>M</italic> phosphate buffer) and at 45°C in a batch system in which parallel separate experiments were performed independently. An IgG adsorption capacity value of up to 140 mg/g was obtained for the HA microcomposite. The IgG molecules could be repeatedly adsorbed and desorbed through the use of spherical microcomposites without a noticeable loss in the IgG adsorption capacity. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 2764–2771, 2013</p> </abstract>