Synthesis of star polymer poly(ethylene glycol)3–poly(N, N‐dimethyl acrylamide) and its application in protein resistance and separation. Issue 3 (27th November 2012)

• Record Type: Journal Article
• Authors: Xing, Jinxing; Tan, Lin; Cao, Fuhu; Wang, Yanmei
• Is Part Of: Journal of applied polymer science. Volume 129:Issue 3(2013:Aug. 05)
• Page Start: 1179
• Page End: 1186
• Publication Date: 2012-11-27
• Subjects: Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.38559
• Languages: English
• ISSNs: 0021-8995

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A star polymer composed of three poly(ethylene glycol) (PEG) arms and one poly(<italic>N, N</italic>‐dimethyl acrylamide) (PDMA) arm (PEG₃–PDMA) was synthesized by amidation and atom‐transfer radical polymerization. The structure of PEG₃–PDMA was confirmed by ¹H‐NMR and gel permeation chromatography results. The surface adsorption and protein‐resistance behaviors of the star polymer PEG₃–PDMA, diblock copolymer PEG–PDMA, and homopolymer PEG were investigated by a quartz crystal microbalance with dissipation. The results indicate that the PEG₃–PDMA coating could reduce protein adsorption to 13% at least, more effectively than the PEG–PDMA coating; this indicated that the protein‐resistance properties depended on the PEG chain density and surface coverage. If PEG₃–PDMA were to be used as the physical coating in capillary zone electrophoresis, it could yield a well‐suppressed eletroosmotic flow with greater stability and separate proteins with a lower relative standard deviration (RSD) of protein migration time and a higher separation efficiency than a bare fused‐silica capillary in a broad pH range. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013</p> </abstract>