In vitro evaluation of electrospun gelatin‐bioactive glass hybrid scaffolds for bone regeneration. Issue 4 (15th May 2012)

• Record Type: Journal Article
• Authors: Gao, Chunxia; Gao, Qiang; Li, Yadong; Rahaman, Mohamed N.; Teramoto, Akira; Abe, Koji
• Is Part Of: Journal of applied polymer science. Volume 127:Issue 4(2013:Feb. 15)
• Page Start: 2588
• Page End: 2599
• Publication Date: 2012-05-15
• Subjects: Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.37946
• Languages: English
• ISSNs: 0021-8995

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>Organic–inorganic hybrid materials, composed of phases that interact on a nanoscale and a microstructure that mimics the extracellular matrix, can potentially provide attractive scaffolds for bone regeneration. In the present study, hybrid scaffolds of gelatin and bioactive glass (BG) with a fibrous microstructure were prepared by a combined sol–gel and electrospinning technique and evaluated <italic>in vitro</italic>. Structural and chemical analyses showed that the fibers consisted of gelatin and BG that were covalently linked by 3‐glycidoxypropyltrimethoxysilane to form a homogeneous phase. Immersion of the gelatin–BG hybrid scaffolds in a simulated body fluid (SBF) at 37°C resulted in the formation of a hydroxyapatite (HA)‐like material on the surface of the fibers within 12 h, showing the bioactivity of the scaffolds. After 5 days in SBF, the surface of the hybrid scaffolds was completely covered with an HA‐like layer. The gelatin–BG hybrid scaffolds had a tensile strength of 4.3 ± 1.2 MPa and an elongation to failure of 168 ± 14%, compared to values of 0.5 ± 0.2 MPa and 63 ± 2% for gelatin scaffolds with a similar microstructure. The hybrid scaffolds supported the proliferation of osteoblastic MC3T3‐E1 cells, alkaline phosphatase activity, and mineralization during <italic>in vitro</italic> culture, showing their biocompatibility. The results indicate that these gelatin–BG hybrid scaffolds prepared by a combination of sol–gel processing and electrospinning have potential for application in bone regeneration. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013</p> </abstract>