Effect and Possible Mechanism of Network Between MicroRNAs and RUNX2 Gene on Human Dental Follicle Cells. Issue 2 (February 2014)

• Record Type: Journal Article
• Authors: Chen, Pei; Wei, Dixin; Xie, Baoyi; Ni, Jia; Xuan, Dongying; Zhang, Jincai
• Is Part Of: Journal of cellular biochemistry. Volume 115:Issue 2(2014:Feb.)
• Page Start: 340
• Page End: 348
• Publication Date: 2014-02
• Subjects: Cytochemistry -- Periodicals; 572
• DOI: 10.1002/jcb.24668
• Languages: English
• ISSNs: 0730-2312

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>ABSTRACT</title> <sec id="jcb24668-sec-0001" sec-type="section"> <p>To investigate whether crosstalk between <italic>RUNX2</italic> and miRNAs is involved in tooth eruption regulated by dental follicle cells (DFCs) and the possible molecular mechanism. Blood samples and embedded dental follicles were collected from patients with cleidocranial dysplasia, and <italic>RUNX2</italic> gene mutations were analyzed, then <italic>RUNX2</italic><sup>+/m</sup> DFCs were isolated and identified. The characteristics of <italic>RUNX2</italic><sup>+/m</sup> DFCs were analyzed. The differential expression of miRNAs was detected between the <italic>RUNX2</italic><sup>+/m</sup> DFCs and <italic>RUNX2</italic><sup>+/+</sup> DFCs by microarray, and target genes were predicted by miRGen. miR‐146a was chosen for further investigation, and its effects in DFCs were analyzed by transfecting its mimics and inhibitors, and expression of genes involved in tooth eruption were detected. A novel insertion mutation (c.309_310insTG) of <italic>RUNX2</italic> gene was identified which had an effect on the characteristics of DFCs. Compared with the <italic>RUNX2</italic><sup>+/+</sup> DFCs, there were 69 microRNAs more than twofold up‐regulated and 54 microRNAs more than twofold down‐regulated in the <italic>RUNX2</italic><sup>+/m</sup> DFCs. Among these, miR‐146a decreased significantly in <italic>RUNX</italic>2<sup>+/m</sup> DFCs, and expression of <italic>RUNX2</italic>, <italic>CSF‐1</italic>, <italic>EGFR</italic>, and <italic>OPG</italic> was significantly altered when miR‐146a was overexpressed or inhibited. <italic>RUNX2</italic> gene mutation contributes to the characteristic change of DFCs, and the crosstalk between <italic>RUNX2</italic> gene and miRNAs may be one of the key regulatory mechanisms of differentiation of DFCs. J. Cell. Biochem. 115: 340–348, 2014. © 2013 Wiley Periodicals, Inc.</p> </sec> </abstract>