Chemotype Diversity of Indigenous Dalmatian Sage (Salvia officinalis L.) Populations in Montenegro. Issue 1 (January 2014)

• Record Type: Journal Article
• Authors: Stešević, Danijela; Ristić, Mihailo; Nikolić, Vuko; Nedović, Marijana; Caković, Danka; Šatović, Zlatko
• Is Part Of: Chemistry & biodiversity. Volume 11:Issue 1(2014:Jan.)
• Page Start: 101
• Page End: 114
• Publication Date: 2014-01
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Biodiversity -- Periodicals; 572
• DOI: 10.1002/cbdv.201300233
• Languages: English
• ISSNs: 1612-1872

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>To identify how many chemotypes of <italic>Salvia officinalis</italic> exist in Montenegro, the chemical composition of the essential oils of 12 wild‐growing populations was determined by GC‐FID and GC/MS analyses. Among the 40 identified constituents, the most abundant were <italic>cis</italic>‐thujone (16.98–40.35%), camphor (12.75–35.37%), 1, 8‐cineol (6.40–12.06%), <italic>trans</italic>‐thujone (1.5–10.35%), camphene (2.26–9.97%), borneol (0.97–8.81%), viridiflorol (3.46–7.8%), limonene (1.8–6.47%), <italic>α</italic>‐pinene (1.59–5.46%), and <italic>α</italic>‐humulene (1.77–5.02%). The composition of the essential oils under study did not meet the ISO 9909 requirements, while the oils of populations <italic>P02</italic>–<italic>P04, P09</italic>, and <italic>P10</italic> complied with the <italic>German Drug Codex.</italic> A few of the main essential‐oil constituents appeared to be highly intercorrelated. Strong positive correlations were observed between <italic>α</italic>‐pinene and camphene, camphene and camphor, as well as between <italic>cis</italic>‐thujone and <italic>trans</italic>‐thujone. Strong negative correlations were evidenced between <italic>cis</italic>‐thujone and <italic>α</italic>‐pinene, <italic>cis</italic>‐thujone and champhene, <italic>cis</italic>‐thujone and camphor, as well as between <italic>trans</italic>‐thujone and camphene. Multivariate analyses allowed the grouping of the populations into three distinct chemotypes, <italic>i.e., Chemotype A</italic>, rich in total thujones, <italic>Chemotype B</italic>, with intermediate contents of thujones, <italic>α</italic>‐pinene, camphene, and camphor and high borneol contents, and <italic>Chemotype C</italic>, rich in camphor, camphene, and <italic>α</italic>‐pinene. The chemotypes did not significantly differ in the total essential‐oil content and the <italic>cis</italic>/<italic>trans</italic>‐thujone ratio.</p> </abstract>