NMR HRMAS spectroscopy of lung biopsy samples: Comparison study between human, pig, rat, and mouse metabolomics. Issue 1 (14th February 2013)

• Record Type: Journal Article
• Authors: Benahmed, Malika A.; Elbayed, Karim; Daubeuf, François; Santelmo, Nicola; Frossard, Nelly; Namer, Izzie J.
• Is Part Of: Magnetic resonance in medicine. Volume 71:Issue 1(2014:Jan.)
• Page Start: 35
• Page End: 43
• Publication Date: 2013-02-14
• Subjects: Nuclear magnetic resonance -- Periodicals; Electron paramagnetic resonance -- Periodicals; 616.07548
• DOI: 10.1002/mrm.24658
• Languages: English
• ISSNs: 0740-3194

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="mrm24658-sec-0001" sec-type="section"> <title>Purpose</title> <p>Using the metabolomics by NMR high‐resolution magic angle spinning spectroscopy, we assessed the lung metabolome of various animal species in order to identify the animal model that could be substituted to human lung in studies on fresh lung biopsies.</p> </sec> <sec id="mrm24658-sec-0002" sec-type="section"> <title>Methods</title> <p>The experiments were conducted on intact lung biopsy samples of pig, rat, mouse, and human using a Bruker Advance III 500 spectrometer. Thirty‐five to 39 metabolites were identified and 23 metabolites were quantified. Principal component analysis, partial least‐squares discriminant analysis, and analysis of variance tests were performed in order to compare the metabolic profiles of each animal lung biopsies to those of the human lung.</p> </sec> <sec id="mrm24658-sec-0003" sec-type="section"> <title>Results</title> <p>The metabolic composition between human and pig lung was similar. However, human lung was distinguishable from mouse and rat regarding: Trimethylamine <italic>N</italic>‐oxide and betaïne which were present in rodents but not in human lung, carnitine, and glycerophosphocholine which were present in mouse but not in human lung. Conversely, succinic acid was undetected in rat lung. Furthermore, fatty acids concentration was significantly higher in rodent lungs compared to human lung.</p> </sec> <sec id="mrm24658-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Using the metabolomics by NMR high‐resolution magic angle spinning spectroscopy on lung biopsy, samples allowed to highlight that pig lung seems to be close to human lung as regarding its metabolite composition with more similarities than dissimilarities. Magn Reson Med 71:35–43, 2014. © 2013 Wiley Periodicals, Inc.</p> </sec> </abstract>