Normal‐phase high‐performance counter‐current chromatography for the fractionation of dissolved organic matter from a freshwater source. Issue 1 (12th December 2013)

• Record Type: Journal Article
• Authors: Sandron, Sara; Nesterenko, Pavel N.; McCaul, Margaret V.; Kelleher, Brian; Paull, Brett
• Is Part Of: Journal of separation science. Volume 37:Issue 1/2(2014)
• Page Start: 135
• Page End: 142
• Publication Date: 2013-12-12
• Subjects: Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201300634
• Languages: English
• ISSNs: 1615-9306

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Normal‐phase high‐performance counter‐current chromatography (HPCCC) is used to obtain a preliminary fractionation of components in dissolved organic matter (DOM) from a freshwater source. The HPCCC solvent system involved a normal‐phase approach with water/methanol (1:1) as the lower stationary phase and hexane/ethyl acetate (1:1) as the upper mobile phase. The critical experiment parameters were optimised: revolution speed 1800 rpm and flow rate 0.15 mL/min. Under these conditions 50 μL of a 0.50 mg/mL DOM solution was loaded. The detection wavelength was monitored at 330 nm in order to isolate the main portion of DOM, which includes substances such as carboxyl‐rich alicyclic molecules. By optimising this system it was possible to isolate materials that, according to GC–MS, can be related to molecules with an analogous structural background. Where fraction analysis was not suitable for GC–MS, RP‐HPLC with UV absorbance detection was used, showing unique chromatograms for each fraction at both 210 and 330 nm.</p> </abstract>