Optimized genetic transformation of Zanthoxylum zanthoxyloides by Agrobacterium rhizogenes and the production of chelerythrine and skimmiamine in hairy root cultures. Issue 1 (19th September 2013)

• Record Type: Journal Article
• Authors: Etsè, Kodjo Djidjolé; Aïdam, Atsou V.; Melin, Céline; Blanc, Nathalie; Oudin, Audrey; Courdavault, Vincent; Creche, Joël; Lanoue, Arnaud
• Is Part Of: Engineering in life sciences. Volume 14:Issue 1(2014:Jan.)
• Page Start: 95
• Page End: 99
• Publication Date: 2013-09-19
• Subjects: Bioengineering -- Periodicals; 660.605
• DOI: 10.1002/elsc.201200216
• Languages: English
• ISSNs: 1618-0240

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p> <italic>Zanthoxylum zanthoxyloides</italic> is an endangered African tree producing numerous bioactive substances including antileukemic and antisickling agents. Here, the potential of <italic>Z. zanthoxyloides</italic> hairy root cultures was tested for the production of bioactive substances with limited natural resources. The efficiency of <italic>Agrobacterium rhizogenes</italic> LBA9402‐mediated transformation of leaf material was evaluated using different techniques. An optimal transformation frequency of 77% was obtained after 11 days by inoculating <italic>A. rhizogenes</italic> directly onto the central vein of 14‐week‐old leaves followed by a co‐cultivation period of 3 days. Different treatments in immersion mode (manual wounding, acetosyringone, CaCl<sub>2</sub>, ultrasonication) never exceeded these results. A maximum growth rate of 0.37 cm/day was determined during the exponential phase. Liquid chromatography‐diode array detection analysis showed the presence of skimmiamine, sesamine, chelerythrine, and chelerythrine derivatives in <italic>Z. zanthoxyloides</italic> hairy root lines. The maximum production of skimmiamine and chelerythrine in 28‐day‐old hairy root cultures was 45 ± 2 and 107 ± 4 mg/100 g dry weight, respectively. The present results highlight the potential of <italic>Z. zanthoxyloides</italic> hairy root cultures for the sustainable production of skimmiamine and chelerythrine.</p> </abstract>