CHITIN SYNTHASE B: A MIDGUT‐SPECIFIC GENE INDUCED BY INSECT HORMONES AND INVOLVED IN FOOD INTAKE IN Bombyx mori LARVAE. Issue 1 (13th December 2013)

• Record Type: Journal Article
• Authors: Zhuo, Weiwei; Chu, Feng; Kong, Lingfei; Tao, Hui; Sima, Yanghu; Xu, Shiqing
• Is Part Of: Archives of insect biochemistry and physiology. Volume 85:Issue 1(2014:Jan.)
• Page Start: 36
• Page End: 47
• Publication Date: 2013-12-13
• Subjects: Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21141
• Languages: English
• ISSNs: 0739-4462

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Chitin synthase (CHS) is the key regulatory enzyme in chitin synthesis and excretion in insects, and a specific target of insecticides. We cloned a CHS B gene of Bombyx mori (<italic>BmChsB</italic>) and showed it to be midgut specific, highly expressed during the feeding process in the larva. Knockdown of <italic>BmChsB</italic> expression in the third‐instar larvae increased the number of nonmolting and abnormally molting larvae. Exposure to nikkomycin Z, a CHS inhibitor, reduced the amount of chitin in the peritrophic membrane of molted larvae, whereas abnormally elevated <italic>BmChsB</italic> mRNA levels were readily detected from the end of molting and in the newly molted larvae. Exogenous 20‐hydroxyecdysone (20E) and methoprene, a juvenile hormone analogue, significantly upregulated the expression of <italic>BmChsB</italic> when the levels of endogenous molting hormone (MH) were low and the levels of endogenous juvenile hormone (JH) were high immediately after molting. When levels of endogenous MH were high and those of endogenous JH were low during the molting stage, exogenous 20E did not upregulate <italic>BmChsB</italic> expression and exogenous methoprene upregulated it negligibly. When the endogenous hormone levels were low during the mulberry‐leaf intake process, <italic>BmChsB</italic> expression was upregulated by exogenous methoprene. We conclude that the expression of <italic>BmChsB</italic> is regulated by insect hormones, and directly affects the chitin‐synthesis‐dependent form of the peritrophic membrane and protects the food intake and molting process of silkworm larvae.</p> </abstract>