Antigenotoxic activities of the natural dietary coumarins umbelliferone, herniarin and 7-isopentenyloxy coumarin on human lymphocytes exposed to oxidative stress. (April 2014)

• Record Type: Journal Article
• Authors: Rezaee, Ramin; Behravan, Effat; Behravan, Javad; Soltani, Fatemeh; Naderi, Yazdan; Emami, Bahareh; Iranshahi, Mehrdad
• Is Part Of: Drug and chemical toxicology. Volume 37:Number 2(2014:Apr.)
• Page Start: 144
• Page End: 148
• Publication Date: 2014-04
• Subjects: Toxicology -- Periodicals; Drugs -- Toxicology -- Periodicals; Toxicology, Experimental -- Periodicals; 615.9005
• DOI: 10.3109/01480545.2013.834352
• Languages: English
• ISSNs: 0148-0545

Abstract

<abstract> <title>Abstract</title> <p>The antigenotoxic effects of umbelliferone (UMB), herniarin (HER) and 7-isopentenyloxy coumarin (7-IP), common natural dietary coumarins, were evaluated on the human lymphocyte DNA damage using single-cell gel electrophoresis. H₂O₂-induced DNA break was measured based on the percentage of DNA in tail, and the antigenotoxic effects of the tested compounds were compared with that of ascorbic acid (10, 25, 50, 100 and 200 μM). UMB, HER and 7-IP did not show any genotoxicity, as compared to phosphate-buffered saline. Treatment with UMB, HER and 7-IP led to a significant reduction in the percentage of DNA in tail induced by H₂O₂ (<italic>p</italic> &lt; 0.001) at all concentrations. The presence of prenyl moiety in the chemical structure of 7-IP may contribute to its better antigenotoxic property, compared to UMB. The results of this study showed that 7-IP possessed the best antigenotoxic activity among the tested compounds.</p> </abstract>