Hepatoprotective role of Abelmoschus esculentus (Linn.) Moench., on carbon tetrachloride-induced liver injury. (September 2013)

• Record Type: Journal Article
• Authors: Saravanan, Subramanian; Pandikumar, Perumal; Pazhanivel, Natesan; Paulraj, Michael Gabriel; Ignacimuthu, Savarimuthu
• Is Part Of: Toxicology mechanisms and methods. Volume 23:Number 7(2013)
• Page Start: 528
• Page End: 536
• Publication Date: 2013-09
• Subjects: Analytical toxicology -- Periodicals; Toxicology -- Periodicals; Toxicology -- Methodology -- Periodicals; 615.907
• DOI: 10.3109/15376516.2013.796032
• Languages: English
• ISSNs: 1537-6516

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Chronic liver disease has become a global health problem. The research for prominent herbal agents for the management of liver diseases is widely increased.</p> <p> <italic>Objective</italic>: The root of <italic>Abelmoschus esculentus</italic> (Linn.) Moench., (Malvaceae) has been used as a remedy for liver disorders. The aim of the present study was to evaluate the antioxidant and hepatoprotective effects of the ethanol extract of <italic>A. esculentus</italic> root.</p> <p> <italic>Materials and method</italic>: The antioxidant effect was assessed using DPPH and hydroxy radical scavenging assays. The hepatoprotective effect of the extract was evaluated using CCl<sub>4</sub> intoxicated HepG<sub>2</sub> cell line and Wistar rats by estimating the levels of hepatic and antioxidant markers.</p> <p> <italic>Results</italic>: The extract of <italic>A. esculentus</italic> showed IC<sub>50</sub> values of 270.99 and 532.86 µg/mL for DPPH and hydroxy radical scavenging assays, respectively. The incubation of HepG2 cells with CCl<sub>4</sub> drastically decreased the cell viability and increased the leakage of transaminases. Pre-treatment with the extract significantly restored the cell death by 31.25 and 39.04% at 200 and 400 µg/mL concentrations, respectively. The reduction of ALT leakage by the treatment was 18.62, 38.59 and 52.15% compared to the CCl<sub>4</sub> treated cells at 100, 200 and 400 µg/mL, respectively. In <italic>in-vivo</italic> experiments also the treatment reduced the levels of transaminases, ALP, MDA, total bilirubin and hepatic TNFα levels as well as increased the antioxidant levels in a dose dependent manner. Histological observations of liver sections showed reduction in steatosis, necrosis and inflammation.</p> <p> <italic>Conclusion</italic>: The results substantiated the hepatoprotective activity of <italic>A. esculentus</italic> through its antioxidant capacity.</p> </abstract>