Microenvironment influence on human colon adenocarcinoma phenotypes and matrix metalloproteinase-2, p53 and β-catenin tumor expressions from identical monoclonal cell tumor in the orthotopic model in athymic nude rats. (March 2014)

• Record Type: Journal Article
• Authors: Priolli, Denise Gonçalves; Abrantes, Ana Margarida; Neves, Silvia; Gonçalves, Ana Cristina; Lopes, Camila Oliveira; Martinez, Natalia Peres; Cardinalli, Izilda Aparecida; Sarmento Ribeiro, Ana Bela; Botelho, Maria Filomena
• Is Part Of: Scandinavian journal of gastroenterology. Volume 49:Number 3(2014)
• Page Start: 309
• Page End: 316
• Publication Date: 2014-03
• Subjects: Gastroenterology -- Periodicals; Digestive organs -- Diseases -- Periodicals; 616.33
• DOI: 10.3109/00365521.2013.869350
• Languages: English
• ISSNs: 0036-5521

Abstract

<abstract> <title>Abstract</title> <p>The present study aims to identify differences between left and right colon adenocarcinoma arising from identical clonal cell and to find out if microenvironment has any influence on matrix metalloproteinase-2 (MMP2), p53 and β-catenin tumor expressions. <bold><italic>Material and methods.</italic></bold> Rats (RNU) were submitted to cecostomy to obtain the orthotopic model of right colon tumor (<italic>n</italic> = 10), while for the left colon model (<italic>n</italic> = 10), a colon diversion and distal mucous fistula in the descending colon was used. Cultivated human colon adenocarcinoma cells (WiDr) were inoculated in stomas submucosa. Histopathological analysis, real-time reverse transcription-PCR for β-catenin, p53 and MMP2, as well as immunohistochemical analysis for p53 and β-catenin expression were conducted. Central tendency, variance analysis and the Livak delta-delta–CT method were used for statistical analysis, adopting a 5% significance level. <bold><italic>Results.</italic></bold> All tumors from the left colon exhibited infiltrative ulceration, while in the right colon tumor growth was predominantly exophytic (67%). In the left colon, tumor growth was undifferentiated (100%), while it was moderately differentiated in the right colon (83%). In right colon tumors, MMP2, p53, and β-catenin gene expressions were higher than compared to left colon (<italic>p</italic> = 4.59354E-05, <italic>p</italic> = 0.0035179, <italic>p</italic> = 0.00093798, respectively, for MMP2, p53 and β-catenin). β-catenin and p53 results obtained by real-time polymerase chain reaction were confirmed by immunohistochemistry assay (<italic>p</italic> = 0.01 and <italic>p</italic> = 0.001, respectively, for β-catenin and p53). <bold><italic>Conclusion.</italic></bold> Left and right human colon adenocarcinomas developed in animal models have distinct phenotypes even when they have the same clonal origin. Microenvironment has influenced p53, β-catenin, and MMP2 expression in animal models of colon cancer.</p> </abstract>