Accelerated sample preparation and formation of astragaloside IV in Astragali Radix. (April 2014)

• Record Type: Journal Article
• Authors: Monschein, Marlene; Ardjomand-Woelkart, Karin; Rieder, Johann; Wolf, Isabella; Heydel, Barbara; Kunert, Olaf; Heuberger, Heidi; Bauer, Rudolf
• Is Part Of: Pharmaceutical biology. Volume 52:Number 4(2014:Apr.)
• Page Start: 403
• Page End: 409
• Publication Date: 2014-04
• Subjects: Pharmacognosy -- Periodicals; Materia medica, Vegetable -- Periodicals; 615.321
• DOI: 10.3109/13880209.2013.839712
• Languages: English
• ISSNs: 1388-0209

Abstract

<abstract> <title>Abstract</title> <p> <italic>Context</italic>: Astragali Radix (Huangqi; <italic>Astragalus mongholicus</italic> BUNGE, Fabaceae) is used in herbal medicinal products as well as in many food supplements. In traditional Chinese medicine, the roots are used for its Qi tonifying, immunostimulant, cardioprotective, hepatoprotective and hypoglycemic effects.</p> <p> <italic>Objective</italic>: Astragaloside IV (AGS-IV), a cycloartane-type triterpene glycoside is used as a marker compound for the quality control of Astragali Radix in various pharmacopoeias.</p> <p> <italic>Materials and methods</italic>: In this study, we analyzed the content of AGS-IV and other astragalosides in various commercial samples of Huangqi by reversed-phase HPLC using evaporative light scattering detection.</p> <p> <italic>Results</italic>: The analyses revealed that AGS-IV is formed during sample preparation from acylated astragalosides like astragaloside I and astragaloside II, when using the assay method of the European Pharmacopoeia.</p> <p> <italic>Discussion and conclusion</italic>: For consistent assay results, the extraction methods of the pharmacopoeias should be re-evaluated and optimized. Alternatively, the hydrolysis by ammonia could be omitted and the genuine compounds like astragaloside I, II and malonyl-AGS-I could be considered for the quality control of Astragali Radix.</p> </abstract>