Insulin Exerts Direct Effects on Carcinogenic Transformation of Human Endometrial Organotypic Cultures. (16th March 2014)

• Record Type: Journal Article
• Authors: Bishop, Erin A.; Lightfoot, Stan; Thavathiru, Elangovan; Benbrook, Doris Mangiaracina
• Is Part Of: Cancer investigation. Volume 32:Number 3(2014)
• Page Start: 63
• Page End: 70
• Publication Date: 2014-03-16
• Subjects: Cancer -- Periodicals; Oncology -- Periodicals; Medical Oncology -- Periodicals; Neoplasms -- Periodicals; 616.994
• DOI: 10.3109/07357907.2013.877479
• Languages: English
• ISSNs: 0735-7907

Abstract

<abstract> <title> <x xml:space="preserve">Abstract</x> </title> <p>Epidemiological studies suggest an association between elevated insulin levels and endometrial cancer. We studied the effects of insulin on normal endometrial cell proliferation with cytotoxicity assays. Organotypic cultures were used to determine the effects of insulin on the development of malignant histological features and anchorage independent growth. Western Blots were used to analyze the mitogen-activated protein kinases and AKT pathways. We found that insulin exerts direct effects on endometrial cells by increasing proliferation and promoting carcinogenesis. Our results suggest that this occurs through ERK 1/2 and glycogen synthase kinase-3β Ser9 phosphorylation.</p> </abstract>