The cellulolytic system of Thermobifida fusca. (August 2014)

• Record Type: Journal Article
• Authors: Gomez del Pulgar, Eva Maria; Saadeddin, Anas
• Is Part Of: Critical reviews in microbiology. Volume 40:Number 3(2014:Aug.)
• Page Start: 236
• Page End: 247
• Publication Date: 2014-08
• Subjects: Microbiology -- Periodicals; Microbiology -- Periodicals; Review Literature -- Periodicals; 579
• DOI: 10.3109/1040841X.2013.776512
• Languages: English
• ISSNs: 1040-841X

Abstract

<abstract> <title>Abstract</title> <p>The process of bioethanol production from biomass comprises pretreatments and enzyme-mediated hydrolysis to convert lignocellulose into fermentable sugars. Because of the recalcitrant character of cellulose, the enzymatic hydrolysis is considered the major challenge in this process to be economically competitive. These technical difficulties highlight the need for the discovery of new enzymes to optimize and lower the cost of current technologies. Microorganisms have developed efficient systems for cellulose degradation. Among cellulolytic microbes, <italic>Thermobifida fusca</italic> possesses great physiological and cellulolytic characteristics (thermostability, high activity and tolerance to a broad pH range) making it an interesting organism to be studied from an applied perspective. In this review we describe the main enzymes/proteins produced by <italic>T.fusca</italic> (cellulases, xylanases, mannanase, manosidase, CBM33 and CelR), the effect of substrate on <italic>T. fusca</italic> proteome, enzyme improvement approaches, synergism between enzymes/proteins and artificial cellulosomes.</p> </abstract>