A novel membrane‐bound E3 ubiquitin ligase enhances the thermal resistance in plants. Issue 1 (16th September 2013)

• Record Type: Journal Article
• Authors: Liu, Zhi‐Bin; Wang, Jian‐Mei; Yang, Feng‐Xi; Yang, Liang; Yue, Yu‐Fei; Xiang, Jun‐Bei; Gao, Mei; Xiong, Fang‐Jian; Lv, Dong; Wu, Xian‐Jun; Liu, Ning; Zhang, Xun; Li, Xu‐Feng; Yang, Yi
• Is Part Of: Plant biotechnology journal. Volume 12:Issue 1(2014:Jan.)
• Page Start: 93
• Page End: 104
• Publication Date: 2013-09-16
• Subjects: Plant biotechnology -- Periodicals; Plant genetic engineering -- Periodicals; 630.272
• DOI: 10.1111/pbi.12120
• Languages: English
• ISSNs: 1467-7644

Abstract

<abstract abstract-type="main" id="pbi12120-abs-0001"> <title>Summary</title> <p>High temperature stress disturbs cellular homoeostasis and results in a severe retardation in crop growth and development. Thus, it is important to reveal the mechanism of plants coping with heat stress. In this study, a novel gene that we identified from <italic>Brassica napus</italic>, referred to as <italic>BnTR1</italic>, was found to play a key role in heat stress response in <italic>planta</italic>. BnTR1 is a membrane‐bound RINGv (C₄HC₃) protein that displays E3 ligase activity <italic>in vitro</italic>. We demonstrated that modest expression of BnTR1 is sufficient to minimize adverse environmental influence and confers thermal resistance on development without any detrimental effects in <italic>B. napus</italic> and <italic>Oryza sativa</italic>. Our investigation into the action mechanism indicates that BnTR1 is likely to be involved in mediating Ca²⁺ dynamics by regulating the activity of calcium channels, which further alters the transcripts of heat shock factors and heat shock proteins contributing to plant thermotolerance. Hence, our study identified BnTR1 as a novel key factor underlying a conserved mechanism conferring thermal resistance in plants.</p> </abstract>