Identification of a novel component C2ORF3 in the lariat–intron complex: lack of C2ORF3 interferes with pre‐mRNA splicing via intron turnover pathway. (4th December 2013)

• Record Type: Journal Article
• Authors: Yoshimoto, Rei; Okawa, Katsuya; Yoshida, Minoru; Ohno, Mutsuhito; Kataoka, Naoyuki
• Is Part Of: Genes to cells. Volume 19:Number 1(2014:Jan.)
• Page Start: 78
• Page End: 87
• Publication Date: 2013-12-04
• Subjects: Cytogenetics -- Periodicals; Cells -- Mechanical properties -- Periodicals; Molecular genetics -- Periodicals; Genes -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Biomechanics -- Periodicals; 571.6
• DOI: 10.1111/gtc.12114
• Languages: English
• ISSNs: 1356-9597

Abstract

<abstract abstract-type="main" id="gtc12114-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p>To identify the novel factors involved in the postsplicing intron turnover pathway, we carried out immunoprecipitation with known postsplicing factors, hPrp43 and TFIP11. As an interacting factor, we identified C2ORF3 protein by mass spectrometry. We found that C2ORF3 protein is present in the previously characterized Intron Large (IL) complex with an excised lariat intron. <italic>In vitro</italic> splicing using C2ORF3‐depleted nuclear extracts showed significant repression of splicing, suggesting that C2ORF3 protein is required for pre‐mRNA splicing through its presumable role in efficient intron turnover. Interestingly, C2ORF3 protein is localized in both the nucleoplasm and nucleoli, which suggests a potential function in rRNA processing.</p> </abstract>